pdf35

Ribogospod. nauka Ukr., 2025; 2(72): 136-153
DOI: https://doi.org/10.61976/fsu2025.02.136
UDC [639.3.043.13:636.087.8]:639.371.2

Effect of probiotic “Subalin” on the productive parameters of age-0+ sturgeon hybrid (Acipenseridae) — bester under conditions of thermal load

Yu. Onyshchuk, This email address is being protected from spambots. You need JavaScript enabled to view it. , ORCID ID 0009-0008-3445-0436, Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine, Kyiv
O. Tretiak, This email address is being protected from spambots. You need JavaScript enabled to view it. , ORCID ID 0000-0003-2300-9115, Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine, Kyiv
O. Kolos, This email address is being protected from spambots. You need JavaScript enabled to view it. , ORCID ID 0009-0008-9312-1368, Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine, Kyiv

Purpose. To determine the effect of adding the probiotic “Subalin” to the diet under conditions of elevated water temperature on productive parameters of age-0+ bester.

Methodology. Experimental studies were carried out in the summer-autumn period of 2024 in the conditions of tanks of the “Biosyla” farm created using heated waste water from an energy facility. Hybrid sturgeon Bester at the age of 0+ were grown for 50 days with feeding with feed of recommended formulations with a crude protein content of 54% and crude fat of 15% with the addition of the probiotic preparation “Subalin” to the diet. The studied probiotic was added to the feed at the rate (% of the feed weight): 0.04 (variant 1) and 0.03 (variant 2). For control, an identical feed without the addition of a probiotic was used. The stocking density of fish with an average body weight of 70.5 g at the beginning of the studies (second decade of August) was 100 ind. per tank (56 ind./m2). The assessment of growth, survival, and productivity parameters of bester and the analysis of physicochemical factors of the aquatic environment were carried out using methods common in fish farming.

Findings. The water temperature in tanks with juvenile bester during the experiments ranged from 28.1 to 33.7°C, which was 5.0 to 5.5°C higher than during the same period in water bodies with the natural temperature regime of the Forest-Steppe zone. The oxygen concentration in the water was within 3.5 to 5.4 mg O2/dm3. Other hydrochemical parameters in the tanks did not significantly exceed the standard values ​​and were typical for water bodies in the region. As a result, after 50 days of cultivation, age-0+ bester at the end of the third decade of September reached an average weight of 248.5 g in the variant with a share of the “Subalin” in the feed of 0.04% and 259.5 g when adding this preparation to the feed in an amount of 0.03%. In the control, the average body weight of the fish was 212.4 g, which was 14.5–18.1% less than in the experimental variants. In both variants of the experiments and the control, 100% survival of fish was recorded. During the entire period of cultivation, the absolute and relative daily gains of the fish of both experimental groups were higher than those of fish of the control group. A preliminary conclusion was made on the feasibility of adding the probiotic “Subalin” to the standard formula feed during the cultivation of age-0+ bester under conditions of heat stress in the amount (% of the feed weight): 0.04 - for fish with an average weight of 20–70 g and 0.03 - for fish with an average weight of more than 70 g.

Originality. The conducted studies on the effect of the probiotic “Subalin” on the productive parameters of age-0+ bester are part of similar studies launched in 2023. In the conditions of sturgeon farming using heated waste water from an energy facility, studies in this area are being carried out for the first time.

Pactical Value. The obtained results are of interest for the development of sturgeon aquaculture in industrial-type farms, in particular at elevated water temperatures in conditions of climate change.

Keywords: sturgeons, bester, pools, feeding, probiotic «Subalin», productivity, water temperature.

REFERENCES

  1. Pashko, S., et al. (2023). The results of obtaining eggs from brood sturgeon (Acipenser baerii Brandt, 1869) at first maturity grown in industrial conditions. Fisheries Science of Ukraine, 4(66), 69-83. https://doi.org/10.61976/fsu2023.04.069.
  2. Pashko, S., et al. (2024). Peculiarities of cultivation of Siberian sturgeon (Acipenser baerii Brandt, 1869) breeders using floating cages in the climatic conditions of the forest steppe of Ukraine. Fisheries Science of Ukraine, 2(68), 40-57. https://doi.org/10.61976/fsu2024.02.040.
  3. Tretiak, O., et al. (2024). Ecological aspects of using heat waste water of energy facilities for sturgeon (Acipenseridae) aquaculture. Fisheries Science of Ukraine, 3(69), 42-62. https://doi.org/10.61976/fsu2024.03.042.
  4. Bondarchuk, M. Ye., & Khartii, M. V. (2022). Svitovi tendentsii rozvytku rynku ikrianykh tovariv. Efektyvna ekonomika, 2. www.economy.nayka.com.ua. Retrieved from: http://www.economy.nayka.com.ua/?op=1&z=10014. https://doi.org/10.32702/2307-2105-2022.2.77.
  5. Palamarchuk, I. P., et al. (2023). Nutritional value of caviar of Siberian sturgeon in Ukraine. Biotechnologia Acta, 16(1), 67-75. https://doi.org/10.15407/biotech16.01.067.
  6. Rahi, D., et al. (2020). Energy pathways associated with sustained spermatozoon motility in the endangered Siberian sturgeon Acipenser baerii. Journal of Fish Biology, 97(2), 435-443. https://doi.org/10.1111/jfb.14382.
  7. Fopp-Bayat, D., et al. (2020). Gonadal analysis in the F1 progeny of a gynogenetic Siberian sturgeon Acipenser baerii female. Animal Reproduction Science, 221, 106548-106548. https://doi.org/10.1016/j.anireprosci.2020.106548.
  8. Aidos, L., et al. (2020). How different stocking densities affect growth and stress status of Acipenser baerii early stage larvae. Animals (Basel), 10(8), 1289. https://doi.org/10.3390/ani10081289.
  9. Madadi, M., & Falahatkar, B. (2025). Effectiveness of Siberian sturgeon (Acipenser baerii) fertilized eggs enrichment with ascorbic acid on incubation performance, tissue concentration and stress responses in larval stage. Aquaculture, 742701. https://doi.org/10.1016/j.aquaculture.2025.742701.
  10. Aidos, L., et al. (2022). Rearing environment during the endogenous feeding stage of Acipenser baerii. Animals (Basel), 12(17), 2205. https://doi.org/10.3390/ani12172205.
  11. Pashko, M. M., et al. (2018). Rezultaty shtuchnoho vidtvorennia osetrovykh ryb, vyroshchenykh u sadkakh za pryrodnoho temperaturnoho rezhymu vodoim lisostepovoi zony Ukrainy. Rybohospodarska nauka Ukrainy, 3, 39-49. https://doi.org/10.15407/fsu2018.03.039.
  12. Zarantoniello, M., et al. (2021). Physiological responses of Siberian sturgeon (Acipenser baerii) juveniles fed on full-fat insect-based diet in an aquaponic system. Scientific Reports, 11(1), 1057-1113. https://doi.org/10.1038/s41598-020-80379-x.
  13. Sarami Foroshani, N., et al. (2024). Supplementation of juvenile Siberian sturgeon (Acipenser baerii) diet with different levels of selenium: Growth performance, digestive enzyme activities, and intestinal histomorphology. Aquaculture Reports, 38, 102325. https://doi.org/10.1016/j.aqrep.2024.102325.
  14. Ramezani, F., et al. (2021). Supplementation of Siberian sturgeon (Acipenser baerii) diet with barberry (Berberis vulgaris) fruit extract: Growth performance, hemato-biochemical parameters, digestive enzyme activity, and growth-related gene expression. Aquaculture, 540, 736750. https://doi.org/10.1016/j.aquaculture.2021.736750.
  15. Pourmoradkhani, F., et al. (2024). Supplementation of Siberian sturgeon (Acipenser baerii) diet with different zinc sources: Effects on growth performance, digestive enzymes activity, hemato-biochemical parameters, antioxidant response and liver histology. Veterinary Research Communications, 48(2), 797-810. https://doi.org/10.1007/s11259-023-10252-5.
  16. Zabytivskyi, Yu., et al. (2024). Growth potential of sterlet (Acipenser ruthenus Linnaeus, 1758) during the juvenile period using the probiotic preparation «Emprobio». Fisheries Science of Ukraine, 1(67), 102-124. https://doi.org/10.61976/fsu2024.01.102.
  17. Tretiak, O. M., et al. (2010). Stan zapasiv osetrovykh ryb ta rozvytok osetrovoi akvakultury v Ukraini. Rybohospodarska nauka Ukrainy, 4, 4-22.
  18. Onyshchuk, Yu., Tretiak, O., & Kolos, O. (2024). The effect of supplementation of feeds with the probiotic “Subalin” on the productivity parameters sturgeon (Acipenseridae) hybrid bester on the first year of cultivation using warm waste water of a power plant. Fisheries Science of Ukraine, 1, 125-141. https://doi.org/10.61976/fsu2024.01.125.
  19. Pylypenko, Yu. V., et al. (2017). Metody ikhtiolohichnykh doslidzhen. Kherson: Oldi-Plius, 2017. 431 s.
  20. Yevtushenko, M. Yu. (2013). Metodyka doslidzhen u rybnytstvi. Kyiv, 2013.
  21. Nabyvanets, B. Y., et al. (2007). Analitychna khimiia poverkhnevykh vod. Kyiv: Naukova dumka.
  22. Arsan, O. M., et al. (2006). Metody hidroekolohichnykh doslidzhen poverkhnevykh vod. Kyiv: Lohos.
  23. Voda rybohospodarskykh pidpryiemstv. Zahalni vymohy ta normy. (2006). SOU – 05.01.37-385:2006. Kyiv: Ministerstvo ahrarnoi polityky Ukrainy.
  24. Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes (2010). Official Journal, 276, 0033-0079.
  25. Onyshchuk, Yu. V., Tretiak, O. M., & Kolos, O. M. (2024). Do pytannia vyroshchuvannia posadkovoho materialu bestera z vykorystanniam teploi skydnoi vody enerhetychnoho obʼiekta. Suchasni problemy ratsionalnoho vykorystannia vodnykh bioresursiv: VI Mizhnarodna naukovo-praktychna konferentsiia, 9-10 zhovtnia 2024 roku, Kyiv, Ukraina: mater. Kyiv, 167-169. https://doi.org/10.61976/conf.IF-2024-6.
Home2025№ 2/2025 (72)2025_02-136_153-Onyshchuk