pdf35

Ribogospod. nauka Ukr., 2023; 4(66): 3-16
DOI: https://doi.org/10.61976/fsu2023.04.003
UDC [595.384.12:591.1]:551.463

Dependence of linear growth and survival of larvae of the oriental river prawn (Мacrobrachium nipponense De Haan, 1849) on temperature and salinity

P. Shekk, This email address is being protected from spambots. You need JavaScript enabled to view it. , Odesa State Environmental University, Odesa
M. Burhaz, This email address is being protected from spambots. You need JavaScript enabled to view it. , Odesa State Environmental University, Odesa
Yu. Astafurov, This email address is being protected from spambots. You need JavaScript enabled to view it. , Odesa State Environmental University, Odesa

Purpose. Studies of the growth and survival of Oriental river prawn (Macrobrachium nipponense) larvae depending on water temperature and salinity during cultivation.

Methodology. Experimental studies were carried out at the Aquatic Department of Aquatic Bioresources and Aquaculture of the Odesa State Ecological University in 2020-2021. Brood Oriental river prawns (Macrobrachium nipponense) were caught in the lower Dniester river and placed into a 0.8 m3 RAS. The larvae were stocked in separate aquariums with automatically maintained specified temperature regime.

The first series of experiments in fresh water investigated the influence of water temperatures of 20–22, 22–24, 24–26, and 28–31 ℃ on the growth of shrimp larvae (from the 1st stage to the Pl stage). Cultivation in water with a salinity of 5, 7, 12 ‰ was carried out at a temperature of 20–22, 22–24, 28–31 ℃. Ten prawn larvae were measured daily under a binocular microscope (MBS-10) using an eyepiece-micrometer. The stage of larval development and the survival rate were determined.

Water salinity was determined using an ATAGO-100 refractometer, and an Azha-101M thermal oximeter was used to determine the oxygen content in water. Statistical processing of data was carried out in Microsoft Excel.

Findings. It was established that at a temperature of 24–26 ℃, the duration of the transition from one stage of larval development to another in Oriental river prawn (Macrobrachium nipponense) increased as the larvae grew and developed. It lasted 4–5 days in the first stages (1-3), then stages from 6 to 9 days in the later stages.

High temperature stimulated the growth of larvae and accelerated their development. In fresh water at a temperature of 29–31 ℃, the length of postlarvae (Pl) reached 6.69±1.15 mm in 28 days of rearing. Cultivation at high temperature resulted in a significant variation in larval sizes (from 4.4 to 8.6 mm CV–25,27).

At lower temperatures (25-27, 22-24 and 20-22 ℃), the average sizes of prawn postlarvae were smaller (5.83±0.78, 4.56±0.15, 4.43±0.15), and the survival rates increased (46, 49 and 54%, respectively).

Survival of larvae was inversely dependent on temperature r = –0.89. It was minimal (32%) at a temperature of 29–31 ℃. The higher the growing temperature, the lower the output of postlarvae.

Water salinity, like temperature, significantly affected the growth, survival and size uniformity of postlarvae. In water with a salinity of 5‰, postlarvae reached their maximum length (6.56±0.15 mm) at a temperature of 29-31 ℃. At temperatures of 20-22 and 22-24 ℃, the size of the postlarvae did not reliably differ among themselves. The maximum survival of postlarvae (52%) was noted at a temperature of 22-24 ℃. Regardless of water temperature, salinity of 5‰ ensured high uniformity of larvae size (CV: 6.11–9.09).

  At a salinity of 7‰, the maximum length of postlarvae was reached at a temperature of 29-31 ℃, and the highest survival rate of prelarvae (34%) was noted at a temperature of 20-22 ℃. The size of the larvae and their survival at other growing temperature conditions did not differ significantly (Р<95). A salinity of 7‰ also ensured the uniformity of postlarvae sizes (CV: 6.12-8.97).

At a salinity of 12‰, high water temperature stimulated the growth of larvae with relative uniformity of their linear sizes, but in all variants of the experiment the survival rate of postlarvae was very low (4–9%).

Originality. Experimental data on the growth and survival of larvae of the Oriental river prawn (Macrobrachium nipponense) depending on temperature and salinity are presented for the first time. The influence of the salinity of the environment on the size uniformity of the larvae was established.

Practical value. The obtained results can be used for the development and improvement of methods of artificial reproduction and cultivation of the Oriental river prawn (Macrobrachium nipponense) under controlled conditions in recirculation aquaculture systems (RAS).

Keywords: prawn Macrobranchium nipponense, rearing, larvae, postlarvae, linear growth, survival, temperature, salinity, size uniformity.

REFERENCE

  1. Salman, S. D., et al. (2006). The invasion of Macrobrachium nipponense (De Haan, 1849) (Caridea: Palaemonidae) into the Southern Iraqi marshes. Aquat Invasions, 1, 109-115. https://doi.org/10.3391/ai.2006.1.3.2 
  2. Shekk, P., & Astafurov, Yu. (2017). Acclimation and ways of spreading the Eastern freshwater shrimp Macrobrachium nipponense (De Haan, 1849) as valuable and economically profitable foodstuff. 8th Science Assembly Meeting Network of aquaculture centers in Central and Eastern Europe (NACEE): proceed. Daugavpils, 46-48.
  3. Cai, Y., & Shokita, S. (2006). Report on a collection of freshwater shrimps (Crustacea: Decapoda: Caridea) from the Philippines, with descriptions of four new species. Raffles B Zool., 54, 245-270.
  4. New, M. B., & Nair C. M. (2012). Global scale of freshwater prawn farming. Aquaculture Research, 43(7), 960-969. https://doi.org/10.1111/j.1365-2109.2011.03008.x.
  5. Suprunovych, A. V., & Makarov, Yu. N. (1990). Kultyvyruemye bespozvonochnye. Pyshchevye bespozvonochnye: ustrytsy, hrebeshky, raky y krevetky. Kiev: Naukova dumka.
  6. Makarov, Yu. N. (2004). Desiatynohye rakoobraznye. Kiev: Naukova dumka.
  7. Fylypenko, S. Y. (2014). O poiavlenyy presnovodnoi vostochnoi krevetky Macrobranchium nipponense (De Haan, 1849) v Dnestre. Academy of sciences of Moldova. International Symposium dedicated to 75th anniversary of professor Andrei Munteanu : proceed. Chișinău, 206-207.
  8. Kulesh, V. F. (1985). Perspektyvy vyrashchyvanyia subtropycheskoi krevetky Macrobrachium nipponense (De Haan) v vodoemakh-okhladyteliakh TES. Byolohycheskye resursy vodoemov v uslovyiakh antropohennoho vozdeistvyia. Kiev: Naukova dumka, 34-36.
  9. Stepanok, N. A. (2014). Vostochnaia rechnaia krevetka roda Macrobrachium v nyzove Dnestra. Hydrobyolohycheskyi zhurnal, 50, 2, 117-120.
  10. Shekk, P. V. (2021). Raspredelenye, chyslennost, razmerno-massovaia y polovaia struktura populiatsyy krevetky Macrobrachium nipponense nyzhneho Dnestra. Akademyku L.S. Berhu – 145 let: sbornyk nauchnykh statei. Bendery: Eco-TIRAS, 483-487.
  11. Bushuiev, S., Snigirov, S., & Son, Mikhail O., et al. (2023). Expansion of the alien East Asian river prawn Macrobrachium nipponense (De Haan, 1849) in southwestern Ukraine and assessment of its commercial usage prospects. Aquatic Invasions, 18, 2, 231-246. https://doi.org/10.3391/ai.2023.18.2.104092 
  12. Shekk, P. V., & Astafurov, Yu. O. (2019). Mozhlyvict kultyvuvannia skhidnoi subtropichnoi prisnovodnoi krevetky Macrobrachium nipponense (De Haan, 1849) v umovakh nyzhnoho Dnistra. Suchasni problemy teoretychnoi ta praktychnoi ikhtiolohii: XII Mizhnar. ikhtiol. nauk.-prakt. konf.: mater. Dnipro, 232-235.
  13. Timmons M. B., & Ebeling J. M. (2002). Recirculation Aquaculture. NRAC Publication, 01-007.
  14. Turanov, V. F. (2003). Razvedenye y vyrashchyvanye presnovodnoi krevetky Macrobrachium rosenbergii na Yuhe Ukrayny. Rybne hospodarstvo Ukrainy, 3, 4 (26, 27), 47-48.
  15. Wickins, J. F., & Lee, D. O. C. (Eds). (2002). Crustacean Farming Ranching and Culture. Oxford (United Kingdom): Blackwell Science. https://doi.org/10.1002/9780470995082 
  16. Beal, B. F., & Protopopescu, G. C. (2012). Ocean-based nurseries for cultured lobster (Homarus americanus Milne Edwards) postlarvae: field experiments off the coast of eastern maine to examine effects of flow and container size on growth and survival. Journal of Shellfish Research, 31 (1), 177-193.
  17. Shekk, P. V., & Astafurov, Yu. O. (2019). Problema biolohichnykh invazii, vselennia chuzhoridnykh vydiv na prykladi skhidnoi prisnovodnoi krevetky Macrobrachium nipponense (De Haan, 1849). Studentska naukova konferentsiia molodykh vchenykh Odeskoho derzhavnoho ekolohichnoho universytetu: zbirnyk mater., 7. Odessa, 102-103.
  18. Nguyen, A. Q., Phan, P. D., Phan, A. T., Nguyen, T. T., Ly, T. N., & Le, B. P. (2002). Biological characteristics of fresh-water prawn Macrobrachium nipponense in Lac Lake and Ea Nhai Reservoir. Proceedings of the 5th Technical Symposium on Mekong Fisheries, Khon Kaen, Thailand, 11–12 December, 2002. Khon Kaen, 251-268.
  19. Ma, K. Y., et al. (2011). The complete mitochondrial genome of Macrobrachium nipponense. Gene, 487, 160-165. https://doi.org/10.1016/j.gene.2011.07.017 
  20. Ogawa, Y., Hashimoto, H., Kakuda, S., & Gushima, K. (1994). On the growth and life span of the population of oriental river prawn Macrobrachium nipponense (De Haan) in the Ashida river (Hiroshima, Japan). Journal of the Faculty of Applied Biological Science – Hiroshima University, 30(1), 43-53.
  21. Mashiko, K. (1990). Diversified egg and clutch sizes among local populations of the freshwater prawn Macrobrachium nipponense. Journal of Crustacean Biology, 10, 306-314. https://doi.org/10.2307/1548489 
  22. Rodriguez, G. (1982). Fresh-water shrimps (Crustacea, Decapoda, Natantia) of the Orinoco Basin and the Venezuelan Guayana. Journal of Crustacean Biology, 2, 378-391. https://doi.org/10.2307/1548054 
  23. Shokita, S. (1979). The distribution and speciation of the inland water shrimps and prawns from the Ryukyu Islands II. Bulletin of the College of Science, University of Ryukyus, 28, 193-278.
  24. Yu, H., & Miyake, S. (1972). Five species of the genus Macrobrachium (Crustacea, Decapoda, Palaemonidae) from Taiwan. Ohmu, 3, 45-55.