Ribogospod. nauka Ukr., 2019; 4(50): 95-108
DOI: https://doi.org/10.15407/fsu2019.04.095 
УДК [639.3.043.13:636.087.8] : 639.371.52

Productive indices of age-2 carp after application of a prebiotic in their feeding

O. Dobryanska, This email address is being protected from spambots. You need JavaScript enabled to view it. , Lviv Research Station of the Institute of Fisheries of the NAAS of Ukraine, Velykyj Lubin
O. Deren, This email address is being protected from spambots. You need JavaScript enabled to view it. , Institute of Fisheries of the NAAS of Ukraine, Kyiv
T. Hryhorenko, This email address is being protected from spambots. You need JavaScript enabled to view it. , Institute of Fisheries of the NAAS of Ukraine, Kyiv

Purpose. To investigate the efficiency of the supplementation of the basic diet of age-3 carp with the prebiotic "Actigen" in pond conditions. To provide control over the aquatic environment conditions, to determine the effect of the tested supplement on the fish productivity of the experimental ponds and feed costs for fish cultivation.

Methodology. Experimental studies were conducted at the fish farm “Karpatskiy Vodograi” LLC of the Pustomyty district of Lviv region under the conditions of similar ponds with one source of water supply. Ponds were stocked with scaly carp yearlings with an average weight of 55 - 58 g, at a stocking density of 1000 fish/ha. The control group of fish was fed with a balanced compound feed, while three experimental groups were supplemented with 0.025% “Actigen” by granulation using the granulation method (Experiment 1); 0.05% (Experiment 2) and 0.075% (Experiment 3). The duration of the study was 60 days. Hydrochemical, hydrobiological and oxygen regimes of the aquatic environment were monitored during the growing season. Hydrochemical and hydrobiological studies were conducted according to the methods described by Alyokin O. A, Zhadin V. I. and Krazhan S. A.

Findings. Satisfactory conditions for carp cultivation were ensured during the growing season. The main parameters of the chemical composition of pond water were in accordance with the fishery standards, there were no peculiarities of changes in these parameters depending on the composition of fish diet. The dissolved oxygen concentration in water ranged from 3.8 to 8.8 mg/dm3. Quantitative development of zooplankton during the growing season was characterized by average parameters. The total number of zooplankton in the ponds ranged from 26.0 to 426.0 thousand ind./m3, and the biomass - from 2.20 to 11.19 g/m3.

It was found that the use of the prebiotic Aktiben prebiotic for feeding age-2 carp at the rate of 0.05%, 0.025% and 0.075% had a positive effect on the fish productivity in the experimental ponds: the average fish weight exceeded the values of the control group by 6.3%, 21.3% and 1.3%, respectively. fish productivity increased by 10.0%, 31.0% and 1.2%. In the same time, the feed costs for carp cultivation decreased by 1.1 (0.025% of the supplement) and 1.3 times (0.05% of the supplement) relative to control.

Originality. For the first time, the effect of the prebiotic action of "Actigen" was investigated, when it was introduced into the basic diet of age-2 carp on productive indices and feed costs.

Practical value. The obtained results indicate the feasibility of using Actigen in aquaculture and the prospect of further studies of its effect on the physiological and biochemical parameters of fish body.

Key word: prebiotic, Aktigen, carp, fish productivity, feed costs, hydrochemical regime, hydrobiological regime.


  1. Hritsyniak, I. I., Grinzhevsky, M. V., Tretyak, O. M., Kiva, M. S., & Mruk A. I. (2008). Farmer Fish Farming. Kyiv.
  2. Chernikova, G. Yu., & Ponomarenko, N. P. (2016). Use of prebiotics based on mannan oligosaccharides in feeding broiler chickens. Bulletin of the Agrarian Science of the Black Sea, 2, 2, 155-159.
  3. Chernikova, Ganna, & Procopenko, Nataliia. (2017). Slaughter quality of broiler-chickens by prebiotic Actigen using. Agrobiodiversity, 50-53. https://doi.org/10.15414/agrobiodiversity.2017.2585-8246.50-53 
  4. Merrifield, D., & Ringo, E. (Eds.). (2014). Aquaculture nutrition. Gut health, probiotics and prebiotics. Chichester: Wiley-Blackwell Publishing. https://doi.org/10.1002/9781118897263 
  5. Tarasenko, N. A., & Filippova, E. V. (2014). Briefly on prebiotics: history, classification, preparation, application. Basic research, 6-1. cyberleninka.ru. Retrieved from http:// cyberleninka.ru/article/n/kratko-o-prebiotikah-istoriya-klassifikatsiyapoluchenieprimene-nie.
  6. Spring, P. (2004). Mannan oligosaccharides - effect on the intestinal microflora and animal health. Sat. scientific Alltech publications, 10-15.
  7. Yaroshenko, F., Dvorska, J., & Surai, P. (2003). Mycotoxins in poultry production; problems and solutions. Poutry International, 3, 12-16.
  8. Che, T. M., Song, M., Johnson, R. W., Kelley, K. W., & Van Alstine, W. G. (2011). Actigen improves growth efficiency and immune responses in pigs experimentally infected with PRRS virus. Science and Technology in the Feed Industry: Alltech’s 27th International symposium, May 22-25, 2011 (conclusion of posters presented). Lexington, KY, USA.
  9. Yang, Y., Iji, P. A., Kocher, A., Thomson, E., Mikkelsen, L. L., & Choct, M. (2008). Effects of mannanoligosaccharide in broiler chicken diets on growth performance, energy utilisation, nutrient digestibility, and intestinal microflora. British Poultry Science, 49, 186-194. https://doi.org/10.1080/00071660801998613 
  10. Vovchenko , B. A., Pentylyuk, S. I., & Smorochinsky, O. M. (2012). Influence of biologicals on the productivity of sheep. Tavriysky Nauchnyk Visnyk, 81, 240-245.
  11. Dvorskaya, Yu. (2013). Mannoligosaccharides - an alternative to growth-stimulating antibiotics for pigs. Livestock Today, 5, 38-42.
  12. Tolstoy, Ruslan, Baglaj, Andrew, Tkachik, Vladimir, & Dvorska, Yulia. (2011). "Aktigen" increases the content of immunoglobulins in sow colostrum. Advanced technology, 5-6 (09-10), 50-51.
  13. Melnyk, M. O. (2017). Influence of new prebiotic preparation on sows productivity. Agrarian science and food technologies, 3 (97), 268-274.
  14. Likhach, V. Ya. (2015). Rationale, development and implementation of intensive technological solutions in pig production: dissertation. Doctor’s thesis. Mykolaiv: National Agrarian University.
  15. Prudchenko, D. V. (2012). Performance indicators of pigs in the use of different drugs. Proceedings of the II International Student Scientific Conference: Biological, technological and environmental aspects of production and processing of livestock products, March 14-16, 2012. Kamyanets-Podilsky, 54-56.
  16. Tukhbatov, I. A. (2017). Improving the productive qualities of broiler chickens when used in the diet of mineral and organic feed additives. Doctor’s thesis. Troitsk: FSBEI HE Orenburg State Agrarian University.
  17. Sverinciuc, C., Benţea, M. I., & Sara, A. (2017). The effects of some fodder additives on growth performance of Siberian sturgeon (Acipenser baeri). Agriculture Science and Practice, 1-2, 101-102.
  18. Le Thanh Hung. (2012). Building new aquafeeds: Feeding for health and performance in Tra catfish (Pangasiaodon hypophthalmus). Science and Technology in the Feed Industry: Alltech’s 28th Annual International symposium, May 20-23, (conclusion of posters presented). Lexington, KY, USA.
  19. Kononenko, R., Shevchenko, P., Kondratyuk, V., & Kononenko, I. (2019). Intensive technologies in aquaculture. Kyiv: Center for Educational Literature.
  20. Kondratyuk, V. M., Kryvenok, M. Ya., & Ilchuk, I. I. (2016). Summary of lectures on the discipline "Feeding of fishes". Kyiv.
  21. Alekin, O. A. (1970). Fundamentals of hydrochemistry. Moscow: Gidrometeoizdat.
  22. Zhadin, V. I. (1950). The study of the aquatic fauna of reservoirs. Moscow: Publishing house of the USSR Academy of Sciences.
  23. Krazhan, S. A. (1997). Natural forage base of growing and feeding joints and ways of its improvement (methodical recommendations). Kyiv, 14-16.
  24. Zheltov, Yu. A. (2003). Methodical instructions for conducting experiments on feeding fish. Fisheries, 62, 23-28.
  25. Nabivaniets, B. I., Osadchy, V. I., Osadcha, N. M., & Nabivaniets, Yu. B. (2007). Analytical chemistry of surface waters. Kyiv: Naukova dumka.
  26. Water of fishery enterprises. General requirements and norms. (2006). SOU-05.01.-37-385:2006. Kyiv: Ministry of Agrarian Policy of Ukraine.