Ribogospod. nauka Ukr., 2015; 2(32): 61-68
УДК 574.3: 579.26
BIOLOGICAL ACTIVITY OF SKIN SECRETIONS IN THE PERCIDAE FAMILY OF FISH WITH REGARD TO ERYSIPELOTHRIX RHUSIOPATHIAE BACTERIA
, Institute of Agroecology and Environmental Management of NAAS, Kуiv
Purpose. To determine the effect of skin secretions in Percidae family of fish (Sander lucioperca, Perca fluviatilis) on the cultures of pathogenic E. rhusiopathiae bacteria.
Methodology. Filter paper was placed on the skin of live fish; after 1 minute it was removed, and the extraction of water-soluble components in the rate of 0.1 cm3 of water per 1 cm2 area of the paper was carried out. The resulting solutions of the secretions of fish skin glands were sterilized by vacuum filtration through the filters with a pore diameter. The test samples contained E. rhusiopathiae cultures and fish skin secretions in 1:10, 1:100, 1:1000 and 1:10000 dilutions. The control samples contained a similar ratio of sterile water and cultures of the experimental bacteria type. The content of E. rhusiopathiae cells in the experimental and control samples was determined after 48 hours, the samples were stored at a temperature of +18... +20 °C.
Findings. The presence of water-soluble extracts of skin secretions obtained from Percidae family of freshwater fish in the environment causes an increase in the density of E. rhusiopathiae bacteria cultures. The degree of the detected stimulating effect of E. rhusiopathiae directly depend on the concentration of the secretions of fish skin glands in the experimental samples. Under the conditions of freshwater ecosystems, such fish as S. lucioperca and P. fluviatilis and E. rhusiopathiae bacteria may form biocenotical relations of the trophic, topical and phoric types.
Originality. The quantitative data proving the biological activity of skin secretions in Percidae family of fish with regard to E. rhusiopathiae pathogenic bacteria have been obtained for the first time.
Practical Value. One of the factors contributing to E. rhusiopathiae bacteria staying in freshwater conditions for a long time can be their biocenotical relations with the components of freshwater biocenoses, including such fish as S. lucioperca and P. fluviatilis. When carrying out measures aimed at preventing and eliminating the outbreaks of infections caused by E. rhusiopathiae, it is necessary to consider the possibility of the persistence of these pathogenic bacteria on the skin of freshwater fish.
Key words: Sander lucioperca, Perca fluviatilis, skin secretions, biological activity, Erysipelothrix rhusiopathiae, stimulating effect.
1. Litvin, V. Y., Ginzgurg, A. L., & Pushkareva, V. I. (1998). Epidemiologytshiskiye aspekty ekologyi bacteriy. Moskva: Farmarus-Print.
2. Borisovіch, Yu. F., & Kirilov, L. V. (1987). Infektsionye bolezni zhivotnykh: Spravochnyk. Moskva: Agropromizdat.
3. Cussler, K., & Balks, E. (2001). 100 years of erysipelas prophylaxis: significance and reduction of animal experiments. ALTEX, 18, 2933.
4. Yamamoto, K., Kijima,M.,& Takahashi, T. (1999). Serovar, pathogenicity and antimicrobial susceptibility of Erysipelothrix rhusiopathiae isolates from farmed wild boars (Sus scrofa) affected with septicemic erysipelas in Japan. Res. Vet. Sci., 67, 301-303. http://dx.doi.org/10.1053/rvsc.1999.0311
5. Wood, R.L., & Beran, G. W. (1994). Erysipelothrix infections. Handbook of Zoonoses. CRC Press, 83-91.
6. Opriessnig, T., Shen, H. G., &Bender, J. S. (2013). Erysipelothrix rhusiopathiae isolates recovered from fish, a harbour seal (Phoca vitulina) and the marine environment are capable of inducing characteristic cutaneous lesions in pigs. Journal of Comparative Pathology, 148 (4), 365-372. http://dx.doi.org/10.1016/j.jcpa.2012.08.004
7. Lehane, L., & Rawlin, G. T. (2000). Topically acquired bacterial zoonoses from fish. Medical Journal of Australia, 173 (5), 25-29.
8. Grishhenko, L. I., Akbaev, M. Sh., & Vasil'kov, G. V. (1999). Bolezni ryb i osnovy rybovodstva. Moskva: Kolos.
9. Gulay, A. V., & Zhukorskiy, O. M. (2013). Biotychni zviazky patohennykh bakterii Erysipelothrixr husiopathiae ta synozelenyk hvodorostei Microcystispulverea. Biolohiia tvaryn, 15 (3), 9-16.
10. Zhukorskiy, O. M., Gulay, A. V., Gulay, V. V., & Tkachuk, N. P. (2014). Changes in the Population Density of Pathogenic Microorganisms in Response to the Allelopathic Effect of Typha Latifolia. Agricultural sciens and practice, 1, 31-36. http://dx.doi.org/10.15407/agrisp1.01.031
11. Hulai, O. V. (2014) Vplyv shkirnykh vydilen' Rhodeus sericeus na patohennykh bakteriy. Naukovi zapysky Ternopil's'koho natsional'noho pedahohichnoho universytetu imeni Volodymyra Hnatyuka. Seriya: Biolohiya, 2 (59), 30-33.
12. Hulai, O. V., & Zhukors'kyy, O. M. (2014). Vplyv shkirnykh vydilen' ryb rodyny Cyprinidae na populyatsiyi patohennykh bakteriy. Rybohospodars'ka nauka Ukrayiny, 3 (28), 80-87.
13. Movchan, Yu. V. (2008–2009). Ryby Ukrayiny (Taksonomiya, nomenklatura, zauvazhennya). Zbirnyk prats' zoolohichnoho muzeyu, 40, 47-86.
14. Shcherbukha, A. Ya. (1982). Fauna Ukrayiny. T. 8 Ryby. Vyp. 4. Kiev: Nauk. dumka.
15. Brandt, Z. (2003). Analiz dannyh. Statisticheskie i vycheslitel'nye metody dlja nauchnyh rabotnikov i inzhenerov. Moskva: AST.