Ribogospod. nauka Ukr., 2013; 1(23): 80-86
DOI: https://doi.org/10.15407/fsu2013.01.080
УДК 597-12:576.85.08



Yu. Rud,  This email address is being protected from spambots. You need JavaScript enabled to view it. , Institute of Fisheries NAAS, Kyiv

The complex method of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) targeting to the 16S rDNA gene was developed for identification of the most widespread gram-negative bacterias of fish. The specificity and efficiency of flexible oligonucleotide primers for 16S rDNA gene of gram-negative bacteria and also possibility of differentiation of these bacteria by use of the restriction endonuclease sets were shown.

Key words: PCR-RFLP, 16s rRNA, gram-negative (G-) bacteria


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