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Ribogospod. nauka Ukr., 2025; 2(72): 118-135
DOI: https://doi.org/10.61976/fsu2025.02.118
UDC 639.371.15:639.3.03

Reproductive parameters of European grayling (Thymallus thymallus Linnaeus, 1758) adapted to artificial growing conditions

A. Kucheruk, This email address is being protected from spambots. You need JavaScript enabled to view it. , ORCID ID 0000-0001-6721-1054, Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine, Kyiv
A. Mruk, This email address is being protected from spambots. You need JavaScript enabled to view it. , ORCID ID 0000-0001-8636-2720, Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine, Kyiv

Purpose. To investigate reproductive parameters, namely: fecundity, size and weight of eggs, embryo survival to the “eye” stage in firstly and repeatedly spawning groups of third-generation European grayling grown and adapted to artificial conditions.

Methodology. The work on the reproduction of European grayling was carried out at the State Institution of Trout Hatchery “Lopushno”. Anesthesia of brood fish was carried out with a natural anesthetic - clove oil at the rate of 1 ml/10 L of water. Measurement of length and weight were carried out on live fish. Based on the results of measurements, an analysis of the obtained values was performed and the productive characteristics of brood fish were determined. Fertilization of eggs was carried out by the “semi-dry” method. De-gluing of fertilized eggs was carried out by washing with clean water for two hours.

Findings. The average weight of age-2, 3, and 4 European graylings used in spawning was 82.32 g, 126.5.4 g and 238 g with an average length of 20.7 cm, 23.4 cm and 28.2 cm, respectively. The results we obtained from 2019 to 2021 showed that under the conditions of the Lopushno farm, European grayling spawners mature en masse at age-2, with the average weight of two-year-old females ranging from 65 to 100 g with an average length of 18.0 to 23.5 cm. Ovulation of eggs in first-spawning females occurred without artificial stimulation. The average working fecundity of females was: age-2 – 597 eggs, age-3 – 1309 eggs, age-4 – 2950 eggs. Fertilization of eggs was 50%.

Originality. A comprehensive analysis of productive and reproductive parameters in third-generation brood European grayling grown in artificial conditions was conducted. A number of factors were identified that may influence the development of high-quality eggs during the creation of broodstocks in controlled conditions of fish farms.

Practical Value. The data obtained during the study will be used as the basis for preparing measures for the artificial reproduction of salmonids in the Carpathian region.

Keywords: eggs, females, males, length and weight, working and relative fecundity.

REFERENCES

  1. Kucheruk, A. I., Mruk, A. I., & Buzevych, I. Yu. (2018). Formuvannia remontno-matochnoho stada ta vidtvorennia yevropeiskoho khariusa Thymallus thymallus (Linnaeus) u shtuchnykh umovakh. Rybohospodarska nauka Ukrainy, 3.https://doi.org/10.15407/fsu2018.03.028.
  2. Kucheruk, A. I., & Mruk, A. I. (2022). Tekhnolohichni osoblyvosti shtuchnoho vidtvorennia yevropeiskoho khariusa. Suchasni problemy ratsionalnoho vykorystannia vodnykh bioresursiv: IV Mizhnarodna naukovo-praktychna konferentsiia, m. Kyiv, 26–27 hrudnia 2022 r.: zbirnyk materialiv. Kyiv: PRO FORMAT.
  3. Mruk, A. I., Velykopolskyi, I. Y., & Ustych, V. I. (2012). Yevropeiskyi kharius baseinu richky Tereblia ta aspekty yoho shtuchnoho vidtvorennia. Rybohospodarska nauka Ukrainy, 1, 53-60.
  4. Kucheruk, A. I., Mruk, A. I., & Stratii, K. M. (Comp.). (2023). Metodychni rekomendatsii zi shtuchnoho vidtvorennia lososevykh ryb. Kyiv: Instytut rybnoho hospodarstva NAAN.
  5. Kucheruk, A. I., & Mruk, A. I. (2023). Tekhnolohichna instruktsiia formuvannia domestykovanykh remontno-matochnykh stad lososevykh ryb vumovakh ryborozplidnykiv (na prykladi DU «Rybovodnyi forelevyi zavod «Lopushno»»). Kyiv: Instytut rybnoho hospodarstva NAAN.
  6. Sävilammi, T., Papakostas, S., Leder, E. H., Vøllestad, L. A., Debes, P. V., & Primmer, C. R. (2021). Cytosine methyation patterns suggest a role of methylation in plastic and adaptive responses to temperature in European grayling (Thymallus thymallus) populations. Epigenetics, 16(3), 271-288. https://doi.org/10.1088/1755-1315/1254/1/012093.
  7. Sundt, H., Alfredsen, K., Museth, J., & Forseth, T. (2022). Combining green LiDAR bathymetry, aerial images and telemetry data to derive mesoscale habitat characteristics for European grayling and brown trout in a Norwegian river. Hydrobiologia, 849(2), 509-525. https://doi.org/10.1007/s10750-021-04639-1.
  8. Dadras, H., Dadras, F., Imentai, A., Malinovskyi, O., Pěnka, T., Kolářová, J., & Policar, T. (2023). Fine ultrastructural features of germ cells and spermatozoa during spermatogenesis in the European grayling, Thymallus thymallus (Teleostei, Salmoniformes, Salmonidae). Frontiers in Veterinary Science, 10, 1188479-1188479. http://dx.doi.org/10.3389/fvets.2023.1188479.
  9. Weiss, S. J., Gonçalves, D. V., Secci-Petretto, G., Englmaier, G. K., Gomes-Dos-Santos, A., Denys, G. P. J., Persat, H., Antonov, A., Hahn, C., Taylor, E. B., & Froufe, E. (2021). Global systematic diversity, range distributions, conservation and taxonomic assessments of graylings (Teleostei: Salmonidae; Thymallus spp.). Organisms Diversity & Evolution, 21(1), 25-42. https://doi.org/10.1007/s13127-020-00468-7.
  10. Szmyt, Mariusz, Piech, Przemysław, Panasiewicz, Grzegorz, Niewiadomski, Piotr, & Pietrzak-Fiećko, Renata. (2021). Gonadal steroid fluctuations and reproduction results of European grayling (Thymallus thymallus L.) from broodstock farmed in a recirculation aquaculture system. Animal Reproduction Science, 224, 106648. https://doi.org/10.1016/j.anireprosci.2020.106648.
  11. Bielikova, O., Mruk, А., Kucheruk, A., & Ciampor, Fedor Jr. (2023). Genetic diversity and heterozygosity of European grayling Thymallus thymallus (Linnaeus, 1758) from fishery stock in Ukraine. Aquaculture Europe  International Conference & Exposition, Sep 18 – Sep 21: proceed., 136-137.
  12. Hagelin, A., & Bergman, E. (2021). Competition among juvenile brown trout, grayling, and landlocked atlantic salmon in flumes — predicting effects of interspecific interactions on salmon reintroduction success. Canadian Journal of Fisheries and Aquatic Sciences, 78(3), 332-338. https://doi.org/10.1139/cjfas-2020-0155.
  13. Hauer, C., & Unfer, G. (2021). Spawning activity of European grayling (Thymallus thymallus) driven by interdaily water temperature variations: Case study gr. mühl River/Austria. River Research and Applications, 37(6), 900-906. https://doi.org/10.1002/rra.3816.
  14. Turek, J., Zlabek, V., Velísek, J., Lepic, P., Cervený, D., Randak, T., & Richoux, N. (2018). Influence of geographic origin on post-stocking survival and condition of European grayling (Thymallus thymallus) in a small river. Aquatic Living Resources, 31. https://doi.org/10.1051/alr/201801.
  15. Johnsson, J. I., Brockmark, S., & Näslund, J. J. (2014). Environmental effects on behavioural development consequences for fitness of captive-reared fishes in the wild. Fish Biol., 85(6), 1946-1971. https://doi.org/10.1111/jfb.12547.
  16. Kodela, T., Jesenšek, D., & Sušnik Bajec, S. (2023). Reproduction parameters of hatchery reared grayling (Thymallus thymallus) females. Animal Reproduction Science, 249, 107196-107196. http://dx.doi.org/10.1080/1828051X.2023.2284368.
  17. Kodela, T., Levart, A., & Sušnik Bajec, S. (2023). Comparative biochemical composition of wild and farm origin grayling (Thymallus thymallus) eggs. Italian Journal of Animal Science, 22(1), 1321-1333. http://dx.doi.org/10.1016/j.anireprosci.2023.107196.
  18. Mruk, A., Kucheruk, A., Bielikova, О., & Čiampor, Jr. F.(2024). Current state of the European grayling (Thymallus thymallus L.) in Ukraine and conservation strategy for population support. A review. Journal for Nature Conservation,  81, 126676, 1-11. https://doi.org/10.1016/j.jnc.2024.126676.
  19. Panasiewicz, P., Niewiadomski, G., Pietrzak-Fie´cko, P. (2021). Gonadal steroid uctuations and reproduction  results of European grayling (Thymallus thymallus L.) from broodstock farmed in a  recirculation aquaculture system. Anim. Reprod. Sci., 224, 106648. https://doi.org/10.1016/j.anireprosci.2020.106648.
  20. Turek, J., Horký, P., Zlabek, V., Velísek, J., Slavík, O., & Randak, T. (2012). Recaptureand condition of pond-reared, and hatchery-reared 1 + European grayling stocked inaddition to wild conspecifics in a small river. Knowledge and Management of Aquatic Ecosystems, 405, 10. https://doi.org/10.1051/kmae/201201.
  21. Frunza, O. E., Khuda, L. V., Lazarenko, L. M., Khudyi, O. I., Karpenko, O. V., & Ya Spivak, M. (2023). The usage of probiotic microorganisms in production technology of European grayling fish stock. IOP Conference Series. Earth and Environmental Science, 1254(1), 12093. https://doi.org/10.1088/1755-1315/1254/1/012093.
  22. Witkowski, A., Jesior, M., 2000. Fecundity of river lamprey Lampetra fluviatilis (L.) in Drweca river (Vistula basin, northern Poland). Arch. Pol. Fish., 8, 225-232.
  23. Wootton, R. J. (1990). Reproduction. Ecology of Teleost Fishes. Netherlands, Dordrecht: Springer, 159-195. https://doi.org/10.1007/978.
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