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Ribogospod. nauka Ukr., 2022; 1(59): 87-108
DOI: https://doi.org/10.15407/fsu2022.01.087
УДК 575.15:597-12

Diagnosis of infectious pancreatic necrosis virus (IPNV): Review

M. Nazarenko, This email address is being protected from spambots. You need JavaScript enabled to view it. , Taras Shevchenko national University of Kyiv, ESC Institute of biology and medicine, Kyiv
Y. Rud, This email address is being protected from spambots. You need JavaScript enabled to view it. , Institute of fisheries of NAAS, Kyiv

Purpose. Infectious pancreatic necrosis virus (IPNV) causes highly contagious infectious pancreatic necrosis disease in rainbow trout and other salmonids. The limited number of preventive and curative remedies makes diagnosis and monitoring the only appropriate way to control the disease. Therefore, the aim of our work was to analyze the literature on methods of virus identification.

Findings. The article presents data on some methods of diagnosis and study of the pathogenesis of infectious pancreatic necrosis. Emphasis is placed on modern molecular biological methods and sets of methods that are recommended for use in the procedure of disease surveillance, including the method of cell culture. Information on polymerase chain reaction methods is widely covered, both in classical modification (RT-PCR) and in real time PCR. Attention is also paid to the latest techniques, such as biosensors and loop isothermal amplification (LAMP).

Originality. Data on the diagnosis and study of infectious pancreatic necrosis virus by modern molecular biological methods and disease monitoring are summarized. Promising ways to identify the virus in aquaculture farms in situ (in the field) are briefly presented.

Practical value. The presented information will help fish pathologists to choose the appropriate method for diagnosing the disease, selection of constituent reactions or a combination of several techniques to improve sensitivity.

Key words. IPNV, diagnostics, RT-PCR, cell culture, biosensors.

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