Ribogospod. nauka Ukr., 2017; 3(41): 83-97
DOI: https://doi.org/10.15407/fsu2017.03.083
УДК: 639.212./.3.034.2:502.7
OPTIMIZATION OF THE CONDITIONS OF STERLET (ACIPENSER RUTHENUS, L. 1758) SPERM CRYOPRESERVATION FOR EGG FERTILIZATION IN FISH FARM CONDITIONS
I. Kononenko,
This email address is being protected from spambots. You need JavaScript enabled to view it.
, National University of Life and Environmental Sciences of Ukraine, Kyiv
A. Pugovkin,
This email address is being protected from spambots. You need JavaScript enabled to view it.
, Institute of Cryobiology and Cryomedicine Problems NAS of Ukraine, Kharkiv
R. Kononenko,
This email address is being protected from spambots. You need JavaScript enabled to view it.
, National University of Life and Environmental Sciences of Ukraine, Kyiv
V. Cherepnin,
This email address is being protected from spambots. You need JavaScript enabled to view it.
, Institute of Fisheries of the NAAS of Ukraine, Kyiv
K. Buts’kyy,
This email address is being protected from spambots. You need JavaScript enabled to view it.
, Institute of Cryobiology and Cryomedicine Problems NAS of Ukraine, Kharkiv
Ye. Kopeika,
This email address is being protected from spambots. You need JavaScript enabled to view it.
, Institute of Cryobiology and Cryomedicine Problems NAS of Ukraine, Kharkiv
Purpose. To optimize the conditions of sterlet sperm cryopreservation for works on egg fertilization and for producing viable offspring in the conditions of sturgeon farms. To analyze the effect of sperm freezing in different forms on spermatozoa survival and egg fertilization. To compare the results of cryoprotective solutions with compositions on the results of their use of sterlet sperm fertilization and to analyze their effect on the quality of the produced offspring.
Methodology. The task aimed at optimizing the conditions of sterlet sperm cryopreservation for egg fertilization in the conditions of fish farms were carried out according to generally accepted methods in cryobiology and recommendations of Ye.F. Kopeika. The works with the produced sterlet offspring were carried out according to conventional sturgeon culture methods.
Findings. The studies showed that the optimization of the conditions of sterlet sperm cryopreservation allowed preserving the properties of thawed spermatozoa at the level of native sperm. It was found that the functions of the sperm cryopreserved in liquid nitrogen vapors in granules in solutions with methanol or dimethyl sulfoxide (DMSO) were better preserved in granules when using methanol. The functions of the sperm after its freezing in cryopreservation solutions №№ 5 and 6 were evaluated in the conditions of a fish farm. It was found that the fertilizing capacity of the cryopreserved sperm in the solution № 5 (85.6 %) (with lower osmolality) did not virtually differ from the control (87.6 %) when using 25-60 g of eggs. In the cryopreservation solution № 5, this value was 17.6 % lower. The weight and length gain after egg fertilization with the sperm cryopreserved in both solutions in 3-month fish was higher than in the control.
Originality. Introduction of creatine and fructose into the cryopreservation solution allowed increasing its protective properties and preserving spermatozoa activity after thawing at the level of the native sperm. Sperm cryopreservation in granules allowed optimizing the conditions of low temperature freezing of this sturgeon species and producing high quality viable offspring.
Practical value. The obtained results can be used by sturgeon culturists in the conditions of fish farms for works aimed at producing viable sterlet offspring both for commercial purposes and for restocking into natural water bodies as well as by cryobiologists for works with other fish species.
Key words: cryopreservation, sterlet, sperm, fertilization, granules, ampoules, creatine, Prussian carp serum.
REFERENCES
- Karanova, M. V., & Gahova, Je. N. (2007). Biohimicheskaja strategija vyzhivanija presnovodnogo molljuska Lymnaea stagnalis pri okolonulevyh temperaturah. Zhurnal jevoljucionnoj biohimii i fiziologii, 43, 3, 258-264.
- Drokyn, S.I., & Kopyejka, Ye.F. (2010). Seredovyshhe dlya kriokonservuvannya spermy koropa. Patent of Ukraine. № 49236.
- Horvаth А., Wayman W.R., Urbányi B. et al. (2005). The relationship of the cryoprotectants methanol and dimethylsulfoxide and hyperosmotic extenders on sperm cryopreservation of two North-American sturgeon species. Aquaculture, 247 (1–4), 243-251.
- Stein, H., & Bayrle, H. (1978). Cryopreservation of the sperm of some freshwater teleosts. Ann. Biol. Anim. Bioch. Biophys, 18, 4, 1073-1076. https://doi.org/10.1051/rnd:19780548
- Stoss, J., & Holtz, W. (1983). Cryopreservation of rainbow trout (Salmo gairdneri) sperm. The effect of DMSO concentration and equilibration time on sperm survival, sucrose and KCL as extender components and the osmolality of the thawing solution. Aquaculture, 32, 321-330. https://doi.org/10.1016/0044-8486(83)90229-6
- Kopyejka, Ye. F., Drokin, S. I., & Gordiyenko, Ye. O. et al. (2009). Vplyv osmotychnosti kriozahysnogo seredovyshha, formy ta ob'yemu kontejneriv na vyzhyvannya kriokonservovanyh spermatozoyidiv koropiv Cyprinus carpio. Rybne gospodarstvo, 66, 89-93.
- Kopeika, E., & Kopeika, J. (2008). Variability of sperm quality after cryopreservation in fish. Fish Spermatology. Oxford, U.K., 347-396.
- Harvey, B. (1983). Cryopreservation of Sarotherodon mossambicus spermatozoa. Aquaculture, 32 (3–4), 313-320. https://doi.org/10.1016/0044-8486(83)90228-4
- Harvey, B., Kelley, N. R. & Ashwood-Smith, M. J. (1982). Cryopreservation of zebrafish spermatozoa using methanol. Can. J. Zool., 60 (8), 1867-1870. https://doi.org/10.1139/z82-242
- Aramli, M. S., Kalbassi, M. R., Nazari, R. M., & Sarvi, K. (2015). Relationships between selected sperm characteristics and fertilization success in the beluga sturgeon, Huso huso (Linnaeus, 1758). Veterinarni Medicina, 60 (9), 509-514.
- Glogowski, J., Kolman, R., & Szczepkowski, M. et al. (2002). Fertilising rate of Siberian sturgeon (Acipenser baerii, Brandt) milk cryopreserved with methanol. Aquaculture, 211 (4), 367-373. https://doi.org/10.1016/S0044-8486(02)00003-0
- Lahnsteiner, F., Berger, B., Horvath, A., & Urbanyi, B. (2004). Studies on the semen biology and sperm cryopreservation in the sterlet, Acipenser ruthenus L. Aquaculture Research, 35, 519-528. https://doi.org/10.1111/j.1365-2109.2004.01034.x
- Tsvetkova, L. I., Cosson, J., & Linhart, O., et al. (1996). Motility and fertilizing capacity of fresh and frozen-thawed spermatozoa in sturgeons Acipenser baeri and A. ruthenus. J. of Applied Ichthyology, 12 (2), 107-112. https://doi.org/10.1111/j.1439-0426.1996.tb00071.x
- Urbany, В., Horvath, А., & Kovacs, В. (2004). Successful hybridization of Acipenser species using cryopreserved sperm. Aquaculture International, 12, 47-56. https://doi.org/10.1023/B:AQUI.0000017186.59304.6d
- Pugovkin, A. Yu., & Kopyejka, Ye.F. (2016). Pronyknist plazmatychnyh membran spermatozoyidiv koropa (Cyprinus carpio, L., 1758) dlya molekul vody ta krioprotektoriv na riznyh etapah kriokonservuvannya. Problemy kriobiologii i kriomediciny, 26 (4), 340-348.
- Stoss, J., & Holtz, W. (1981). Cryopreservation of rainbow trout (Salmo gairdneri) sperm. Effect of thawing solution, sperm density and interval between thawing and insemination. Aquaculture, 22, 97-104. https://doi.org/10.1016/0044-8486(81)90136-8
- Horváth, Á., Miskolczi, E., & Mihalffy, S. et al. (2007). Cryopreservation of common carp (Cyprinus carpio) sperm in 1.2 and 5 ml straws and occurence of haploids among larvae produced with cryopreserved sperm. Cryobiology, 54 (3), 251-257.
- Dokina, O. B., Cvetkova, L. I., Pronina, N. D., & Milenko, V. A. (2006). Metod kriokonservacii spermy osetrovih ryb — obektov akvakul'tury. Akvakul'tura osetrovyh ryb: dostizhenija i perspektivy razvitija: IV Mezhdunarodnау nauch-prakt. konf. Moskva: VNIRO, 76-79.
- Dokina, O. B., Cvetkova, L. I., Pronina, N. D., & Milenko, V. A. (2008). Sposob kriokonservirovanija spermy osetrovih ryb. Patent of RF № A01K 61/00, A0N 1/02.
- Shishanova, E. I., Trenkler, I. V., & Mamonova, A. S. (2012). Vlijanie kriokonservacii spermy na vyzhivaemost' i geneticheskij polimorfizm lichinok russkogo osetra. Vestnik AGTU. Ser.: Ryb. hoz-vo, 2, 105-110.
- Dzyuba, B., Cosson, J., & Yamaner, G. et al. (2013). Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa. Cryobiology, 66, 192-194. https://doi.org/10.1016/j.cryobiol.2012.12.003
- Karanova, M. V. (2009). Sostav svobodnyh aminokislot krovi i myshc rotana Perccottus glehni v period podgotovki i zavershenija gibernacii. Zhurnal jevoljucionnoj biohimii i fiziologii, 45 (1), 59-67.
- Stein, H. (1979). Zum Einflus von Schutzsubstanz und Konfektionierung auf das Befruchtungsergebnis mit tiefgefrorenem Bachforellensperma (Salmo trutta morpha fario L.). Berl. Munch. Tierarztliche Wochenschrift, 92, 420-421.
- Lahnsteiner, F., Weismann, T., & Patzner, R. A. (1997). Methanol as cryoprotectant and the suitability of 1.2 ml and 5 ml straws for cryopreservation of semen from salmonid fishes. Aquaculture Research, 28, 471-479.
- Pugovkin, A. Ju., Kononenko, I. S., & Kononenko, R. V., et al. (2016). Kriokonservirovanie spermy sterljadi: optimizacija sostava kriozashhitnoj sredy. Problemy kriobiologii i kriomediciny, 26 (2), 160.
- Mounib M. S. (1978). Cryogenic preservation of fish and mammalian spermatozoa. J. Reprod. Fert., 53 (1), 13-18. https://doi.org/10.1530/jrf.0.0530013
- Mounib, M. S., Hwang, P. C., & Idler, R. R. (1968). Cryogenic preservation of Atlantic Cod (Gadus morhua) sperm. J. Fish. Res. Board. Can., 25 (12), 2623-2632. https://doi.org/10.1139/f68-232
- Milovanov, V. K. (1962). Biologija vosproizvodstva i iskusstvennoe osemenenie zhivotnyh. Moskva: Sel'hozizdat.
- Kopejka, E. F., Cherepanov, V. V., Ajushin, B. N., & Rachek, E. I. (1990). Ispol'zovanie kriokonservirovannoj spermy karpa dlja kommercheskoj gibridizacii na teplyh vodah Primorskoj GRES. Rybohozyaystvennoe ispol'zovaniju teplykh vod: IV Vses. soveshh. Moskva, 145-146.
- Savushkina, S. I. (2007). Vyrashhivanie ryboposadochnogo materiala, poluchennogo s ispol'zovaniem kriokonservirovannoj spermy. Racional'noe ispol'zovanie presnovodnyh jekosistem — perspektivnoe napravlenie realizacii nacional'nogo proekta «Razvitie APK»: nauch.-prakt. konf.: mater. Moskva: Rossel'hozakademija, 303-305.