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Ribogospod. nauka Ukr., 2022; 1(59): 61-74
DOI: https://doi.org/10.15407/fsu2022.01.061
УДК 639.371.52:621

Characteristics of productive-biological parameters of age-1+ carp produced with the use of sperm cryopreservation

D. Syrovatka, This email address is being protected from spambots. You need JavaScript enabled to view it. , Institute of Fisheries NAAS of Ukraine, Kyiv
M. Osipenko, This email address is being protected from spambots. You need JavaScript enabled to view it. , Institute of Fisheries NAAS of Ukraine, Kyiv
H. Kurinenko, This email address is being protected from spambots. You need JavaScript enabled to view it. , Institute of Fisheries NAAS of Ukraine, Kyiv
V. Gurbyk, This email address is being protected from spambots. You need JavaScript enabled to view it. , Institute of Fisheries NAAS of Ukraine, Kyiv
O. Fedonenko, This email address is being protected from spambots. You need JavaScript enabled to view it. , Dnipro State Agrarian-Economical University, Dnipro

Purpose. To create stocks of age-1 and 1+ carp produced with the use of defrosted and native sperm and provide the comprehensive characteristics of their quantitative and qualitative parameters.

Methodology. The material for the study were age-1 and 1+ Nyvky scaly and non-scaly carp. Main quantitative and qualitative parameters were studied during spring assessment and autumn harvest using conventional aquaculture methods. Stocking density of age-0+ fish for wintering was 10 thousand kg/ha, age-1 fish – 1 thousand fish/ha. The exterior assessment of multiple age groups was carried out using following parameters: body weight and length, body depth, body circumference. Measurements were done using a measuring tape with a precision of 1 mm. Fish were weighed individually on electronic balances with a precision of 1 g. Following main exterior indices were determined and analyzed: circumference index, body length to depth ratio, head index.

Findings. The study showed that age-1 fish produced from defrosted sperm had 0.25 and 4.5% higher survivability during wintering with the prevalence of non-scaly carp, however they were characterized by higher weight loss: 1.13% for scaly carp and 1.65% for non-scaly carp. Age-1+ fish produced from defrosted sperm prevailed over their age-0+ by weight by 2.69% for scaly carp and by 7.34% for non-scaly carp. However, fish of the experimental group were characterized by lower survivability by 1.0% and 3.25% compared to the control group. Fish productivity parameters during the study period varied from 810.7 to 1056.0 kg/ha.

Originality. For the first time in Ukraine, a comparative analysis of quantitative and qualitative parameters of age-1 and 1+ carp produced with the use of defrosted and native sperm was performed.

Practical value. Use of the method of cryopreservation of sperm obtained from brood fish with desired peculiarities of genotype allows improving creation of highly productive stocks using methods of individual selection, getting highly productive crosses as well as hybrids between geographically isolated populations.

Key words: carp, defrosted sperm, age-1 fish, age-1+ fish.

REFERENCE

  1. Kopejka, E. F., Pushkar, N. S., & Belous, A. M. (1981). Nizkotemperaturnaya konservaciya spermy ryb. Genetika, selekciya gibridizaciya ryb: 2 Vsesoyuz. soveshchanie, Rostov-na-Donu, 16-20 marta, 105-106.
  2. Piliev, S. A.(1989). Primenenie kriokonservacii v rybovodstve. Selekciya ryb, 100-105.
  3. Rott, H. H. (1994). Ispol’zovanie kriokonservirovannogo geneticheskogo materiala dlya vosstanovleniya redkikh i ischezayushchikh vidov gidrobiontov. Konservaciya genet. resursov, 1, 2-8.
  4. Kononenko, I. S., & Bekh, V. V. (2016). Kriokonservuvannia statevykh produktiv — efektyvnyi metod zberezhennia bioriznomanittia osetrovykh vydiv ryb. Rybohospodarska nauka Ukrainy, 2, 5-21. https://doi.org/10.15407/fsu2016.02.005 
  5. Güneş, Yamaner, Ekici, A., & Devrim, Memiş. (2015). A brief overview on cryopreservation method of sturgeon sperm. Biology. Journal of Fisheries & Aquatic Sciences, 30(2), 4-20. DOI: 10.18864/IUJFAS.11849.
  6. Horvath, A.,et al.(2012). Application of sperm cryopreservation to hatchery and species conservation: A case of the Adriatic grayling (Thymallus thymallus). Aquaculture, 358, 213-215. https://doi.org/10.1016/j.aquaculture.2012.07.012   
  7. Betsy, J., & Kumar, S. (2020). Cryopreservation: History and Development. Cryopreservation of Fish Gametes. Singapore: Springer. https://doi.org/10.1007/978-981-15-4025-7_6.
  8. Babiak, I., & Giogowski, J.(1995). Kriokonserwacja nasienia ryb slodkowodnych – wyniki prac badawczych przeprowadzonych w latach 1993—94 w Polsce. Arch. Rybactwa Рol., 3, supl. 1, 19-28.
  9. Glogowski, J., & Ciereszko, A. (2008). Kriokonserwacja nasienia ryb – znaczenia, specyfika oraz krajowe osiagniecja w ostatnim dziesiecioleciu. Med. Wet., 64,551-554.
  10. Zelinskij, YU. P. (1990). Nekotorye strukturno-populyacionnye i ehkologo-biokhimicheskie problemy kriokonservacii geneticheskikh resursov atlanticheskogo lososya. Simpozium po atlanticheskomu lososyu: tezisy dokl. Syktyvkar, 25.
  11. Cvetkova, L. I. (1998). Sozdanie nizkotemperaturnoj kollekcii spermy ryb. Problemy reproduktivnoj biologii v trudakh professora S. I. Kulaeva i ego posledovatelej. Moskva: MGU, 326-330.
  12. Lahnsteiner, F. (2000). Semen cryopreservation in the Salmonidae and in the Northern pike. Aquac. Res., 31, 245-258. https://doi.org/10.1046/j.1365-2109.2000.00452.x 
  13. Bibenko, O.V.,& Kopejka, E.F. (1992). Vliyanie kriokonservacii spermy karpa na ehmbriogenez. Kriokonservirovanie reproduktivnykh kletok i ehmbrionov. Khar’kov, 3-8.
  14. Cherepnin, V.A., Bezusyj, A.L.,& Syrovatka, D.A. (2014). Ocenka effekta krioselekcii pri ispol’zovanii optimizirovannykh kriozashchitnykh sred dlya zamorazhivaniya spermy karpovykh ryb. Izvestiya vysshikh uchebnykh zavedenij. Ural’skij region. Chelyabinsk, 1,112-117.
  15. Isaksson, A. (1998). The status of Icelandic salmonid resources, with special reference to genetic conservation policy. Action before extinction. Victoria, British Columbia, Canada, 115-127.
  16. O’Reilly, P., & Doyle, R. (2007) Live gene banking of endangered populations of Atlantic salmon. The Atlantic salmon. Genetics, Conservation and Management. Oxford, 425-469. https://doi.org/10.1002/9780470995846.ch14 
  17. Piironen, J., & Heinimaa, P. (1998). Preservation programs for endangered fish stocks in Finland. Action before extinction. Victoria, British Columbia, Canada,105-113.
  18. Instruktsiia z orhanizatsii pleminnoi roboty v koropivnytstvi Ukrainy. Intensyvne rybnytstvo.(1995). Kyiv: Ahrarna nauka, 3-34.
  19. Instruktsiia z orhanizatsii ta vedennia promyslovoi hibrydyzatsii v koropivnytstvi (1995). Intensyvne rybnytstvo. Kyiv: Ahrarna nauka, 74-83.
  20. Demchenko, I. F. (1995). Instruktsiia z orhanizatsii zymivli ryb. Intensyvne rybnytstvo. Kyiv: Ahrarna nauka, 178-185.
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