pdf35

Ribogospod. nauka Ukr., 2017; 3(41): 98-108
DOI: https://doi.org/10.15407/fsu2017.03.098 
УДК: 574.64+597.551.2:591.[1/5]

PATHOMORPHOLOGICAL AND CYTOMETRIC PARAMETERS OF BLOOD RED CELLS OF AGE-2 PRUSSIAN CARP (CARASSIUS AURATUS GIBELIO (BLOCH, 1782) IN THE CONDITIONS OF INTOXICATION WITH COPPER IONS

T. Sharamok, This email address is being protected from spambots. You need JavaScript enabled to view it. , Dnipro National University named after Oles Honchar
N. Yesipova, This email address is being protected from spambots. You need JavaScript enabled to view it. , Dnipro National University named after Oles Honchar
N. Kolesnyk, This email address is being protected from spambots. You need JavaScript enabled to view it. , Institute of Fisheries of the NAAS of Ukraine, Kyiv 

Purpose. To detect the effect of elevated copper ion concentrations (10 aquaculture Maximum Permissible Limits) on morphological and cytometric parameters of erythrocytes of age-2 Prussian carp (Carassius gibelio Bloch, 1782) in experimental and natural conditions.

Methodology. During the work, summarized results of studies performed in 2015-2016 were used. Morphological and cytometric parameters of Prussian carp erythrocytes were determined in the conditions of natural habitats (Zaporizhzhia reservoir) and an experiment. Copper ion concentration both in the experiment and natural conditions was similar and was 0.01 mg/L (10 aquaculture Maximum Permissible Limits). Experimental studies were performed during 21 days. In the control aquarium, fish were kept in the settled tap water; while in the experimental aquaria, intoxication of fish with copper ions was modelled by introducing CuSO4 in water. Blood smears were examined under 40x and 100 x magnifications with the use of microphotography (digital camera Sciencelab T500 5.17 M).

Findings. The performed hematological studies showed that under the conditions of experimental chronic intoxication with copper ions (0.01 mg/L), age-2 Prussian carp had an increase in the share of immature forms of erythrocytes, increase in the number of erythrocytes with pathological signs (cell wall destruction, atypical forms), increase in the nucleus-cytoplasm ration, but the difference in cytometric parameters of erythrocytes between experimental and control fish was not significant. When comparing the morphometric parameters of erythrocytes of fish kept in experimental and natural conditions with similar copper ion concentrations (0.01 mg/L), a significant increase in the nucleus areas of mature erythrocytes was detected and, correspondingly, an increase in the nucleus-cytoplasm ratio of erythrocytes (by almost 30%) in fish in experimental conditions compared to fish, which lived in the Zaporizhzhia reservoir. An increase in these parameters indirectly indicate on the inhibition of erythrocyte functional activity.

Originality. For the first time, an analysis of the effect of copper ions at a concentration of 0.01 mg/L (10 aquaculture Maximum Permissible Limits) on cytometric parameters of Prussian carp erythrocytes in experimental and natural conditions was presented.

Practical value. The obtained results can be used for detecting adaptation reactions in fish organisms under the conditions of the effect of heavy metals as well as for hydroecological monitoring for detecting the levels of heavy metal pollution in aquatic ecosystems dangerous for hydrobionts.

Key words. Prussian carp (Carassius gibelio Bloch, 1782), copper, erythrocytes, pathomorphological and cytometric parameters, Zaporizhzhia reservoir, experiment.

REFERENCES

  1. Arnaudov, A. D., Velcheva, I. G. & Tomova, E. S. (2008). Influence of copper on the erythrocyte-metric parameters of Carassius gibelio (Pisces, Cyprinidae)// Bulgarian Journal of Agricultural Science, 14 (6), 557-563.
  2. Kondera, E. (2011). Wpływ kadmu i miedzi na aktywność krwiotwórczą nerki głowowej karpia. Ochrona Środowiska i Zasobów Naturalnych, 48, 143-150.
  3. Georgieva, E., Arnaudov, A., & Velcheva, I. (2010). Clinical, hematological and morphological studies on ex situinduced copper intoxication in crucian carp (Carassius gibelio). Journal of Central European Agriculture, 11, 2, 165-172.
  4. Sharamok, T. S., & Yesipova, N. B. (2015). Vplyv antropohennykh faktoriv na hematolohichni pokaznyky ryb. Naukovi zapysky Ternopil's'koho nats. ped. un-tu im. V. Hnatyuka, 3-4 (64), 722-726.
  5. Craig, P. M., Wood, C. M., & McClelland, G. B. (2007). Oxidative stress response and gene expression with acute copper exposure in zebrafish (Danio rerio). American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 293, 1882-1892. https://doi.org/10.1152/ajpregu.00383.2007 
  6. Nawaz, M., Manzl, C., & Krumschnabel, G. (2005). In vitro toxicity of copper, cadmium, and chromium to isolated hepatocytes from carp, Cyprinus carpio L. Bulletin of Environmental Contamination and Toxicology, 75, 652-661. https://doi.org/10.1007/s00128-005-0802-0 
  7. Tan, F. X., Wang, M., & Wang, W. M. et al. (2008). Comparative evaluation of the cytotoxicity sensitivity of six fish cell lines to four heavy metals in vitro. Toxicology in Vitro, 22, 164-170. https://doi.org/10.1016/j.tiv.2007.08.020  
  8. Mineev, A. K. (2013). Nespecificheskie reakcii u ryb iz vodoemov srednej i nizhnej. Izvestija Samarskogo nauchnogo centra Rossijskoj akademii nauk, 3-7, 15, 2301-2318.
  9. Gileva, T. A., & Kosticyna, N. V. (2014). Harakteristika perifericheskoj krovi i soderzhanija tjazhjolyh metallov v organah i tkanjah okunja vodojomov bassejna r. Kamy. Teoreticheskaja i prikladnajaj ekologija, 2, 45-50.
  10. Verholyas, M. R., Veyalkina, N. M., & Honcharuk, V. V. (2010).Vplyv ioniv midi na hematolohichni ta tsytohenetychni pokaznyky prisnovodnykh ryb Carassius auratus gibelio. Tsytolohyya y henetyka, 2, 65-70.
  11. Kuzina T.V. (2009). Analiz gematologicheskih pokazatelej sudakaVolgo-Kaspijskogo kanala. Estestvennye nauki. – Astrahan', 4 (29), 96-100.
  12. Mineev, A. K. (2007).Morfologicheskij analiz i patologicheskie izmenenija struktury kletok krovi u ryb Saratovskogo vodohranilishha. Voprosy ihtiologii, 1, 93-100.
  13. Golovina, N. A., & Trombickij, I. D. (1989). Gematologija prudovyh ryb. Kishinev: Shtiinca.
  14. Serpunin, G. G. (2002). Gematologicheskie pokazateli adaptacij ryb. Doctor’s thesis. Kaliningrad.
  15. Sharon, G., & Zilberg, D. (2012). Atlas of Fish Histology and Histopathology. Central and Northen Arava Research and Development Centers.
  16. Yesipova, N. B., & Surova, Yu. O. (2015). Osoblyvosti morfostruktury erytrotsytiv molodi riznykh vydiv ryb v umovakh hipoksiyi. Suchasni problemy teoretychnoyi i praktychnoyi ikhtiolohiyi. Herson: Hrin' D.S., 71-74.
  17. Sharamok, T. S., Yesipova, N. B., & Fedonenko, O. V. et al. (2016). Ekoloho-hematolohichna kharakterystyka plitky zvychaynoyi (Rutilus rutilus Linnaeus1758) Zaporiz'koho vodoskhovyshcha. Biolohichnyy visnyk MDPU imeni Bohdana Khmel'nyts'koho, 6 (2), 303-310.
  18. Ivanova, N. T. (1983). Atlas kletok krovi ryb. Moskva: Legkaja i pishhevaja promyshlenost'.
  19. Nikolov, B., & Boyadzieva-Doichinova, D. (2010). Parameters of the red blood cell count in three species of carp fishes. Bulgarian Journal of Agricultural Science, 16 (3), 307-310.