Ribogospod. nauka Ukr., 2014; 2(28): 69-78
DOI: https://doi.org/10.15407/fsu2014.02.069
УДК 597-12:576.85.08

MOLECULAR DIAGNOSTICS OF YERSINIA RUCKERI

Yu. Rud, This email address is being protected from spambots. You need JavaScript enabled to view it. , Institute of Fisheries of the NAAS, Kyiv
I. Tsyganok, This email address is being protected from spambots. You need JavaScript enabled to view it. , ESC Institute of Biology T. Shevchenko National University of Kyiv, Kyiv

Purpose. The analysis of nucleotide sequences of the 16S rDNA gene of virulent strains of Yersinia ruckeri and to develop the method of molecular diagnostic of enteric redmouth disease.

Methodology. By the method of CLUSTALW algorithm in MEGA software version 6.0 the nucleotide sequences of the 16S rDNA gene of virulent strains of Yersinia ruckeri were analysed. For development of molecular diagnostic of Y. ruckeri the method of polymerase chain reaction (PCR) was used. Primer selection was carried out in software VectorNTI11 and on-line-service BLAST. The PCR products were investigated by the methods of sequencing and nucleotide analysis.

Findings. Based on PCR assay the method of molecular diagnostic of enteric redmouth disease agent, bacterium Y. ruckeri was developed. It was shown that specific oligonucleotide primers generated PCR products in size of 600 base pairs. PCR products were investigated by the sequencing that showed right targeting of primers in reaction.

Originality. Among high-conservative gene of 16S rDNA of Y. ruckeri the fragment of DNA was determined to which the specific primers for rapid diagnostic of virulent strains were selected.

Practical Value. Rapid diagnostic of yersiniosis will allow to identify an agent of this infectious disease, bacterium Y. ruckeri, and to provide the prophylactic or medical measures in the fish farming of Ukraine.

Key words: Y. ruckeri, rapid diagnostic, PCR.

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