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Ribogospod. nauka Ukr., 2018; 2(44): 103-115
DOI: 10.15407/fsu2018.02.103 
УДК [639.3.043:636.087.73]:639.371.52

Determination of the efficiency of the use of amaranth (Amaranthus) in feeding of carp under different rearing conditions

R. Palamarchuk, This email address is being protected from spambots. You need JavaScript enabled to view it. , Institute of Fisheries of the NAAS, Kyiv
O. Deren, This email address is being protected from spambots. You need JavaScript enabled to view it. , Institute of Fisheries of the NAAS, Kyiv

Purpose. Determination of the effect of adding an Amaranthus (Amaranthus) supplement with anti-oxidant properties to the basic diet of carp on some fish-physiological parameters depending on rearing conditions.

Methodology. Aquaculture, ichthyological and hydrochemical studies were conducted according to generally accepted methods in fish culture. The hemoglobin content in carp blood was determined by hemoglobin-cyanide method. The number of red blood cells in the blood of carp was counted in the Goryaev’s chamber. Determination of protein content was carried out using the Bradford’s method. The concentration of diene conjugates was estimated by a method based on the reaction of the optical density of the heptaninopropanol lipid extract. Determination of the concentration of TBK-active products was carried out spectrophotometrically by color reaction with thiobarbituric acid. The activity of superoxide dismutase (SOD) was determined by the inhibition of the reaction of nitrosin tetrazolium reduction in the presence of phenazine methisulphate. The activity of catalase was evaluated based on changing H2O2 concentration.

Findings. The study analyzed the effect of feeding carp with grains of Ukrainian high-yielding feed grade amaranth with healing properties (Kharkiv-1) on physiological and biochemical parameters of fish organism. A comparative description of the efficiency of using this feed supplement under standard conditions of cultivation and the effect of a stress factor (water pollution as a result of intensification measures) was carried out. In both experiments, an increase in the number of formed blood elements was observed in the blood of experimental groups of fish: there was a tendency of an increase in the number of erythrocytes by 15-18.1% compared to control. Under optimum conditions of rearing, there was a tendency for an increase in body fat in the experimental group carp carps, while the effect of stress factors increases the protein (p<0.01) and fat contents compared to control. Under optimal conditions of cultivation, the tendency of an increase in SOD activity, both in muscles and in hepatopancreas of carp (by 5.9-8.3%.) was observed. Under the effect of stress factors, the activity of this enzyme was significantly increased (p˂0.01 ) that indicated on the activation of the anti-oxidant properties of amaranth in the event of negative factors.

Originality. For the first time the effect of biologically active properties of amaranth on some fish and physiological parameters of age-1+ fish was studied and the effectiveness of its use under optimum conditions of cultivation, as well as the effect of stress factor was determined.

Practical value. Laboratory simulation of high content of nitrites in water, as widely common stress-factor in fish farming, and comparison of its effect on some fish-physiological parameters of carp organism is justified and topical. Taking into account positive results of the introduction of this feed additive into the diet of carp, it is possible to solve the problems of fish farms caused by the intensification of production, namely: reduction in fish growth gain and their death due to hypoxia and the development of concomitant diseases as a result of organic pollution of the aquatic environment.

Key words: amaranth, Nyvky carp, age-1+ fish, physiological-biochemical indices of the organism, conditions of cultivation, chemical composition of water.

REFERENCES

  1. Sherman, I. M. (1994). Stavove rybnytstvo. Kyiv: Urozhai.
  2. Hrynzhevskyi, M. V., & Pshenychnyi, D. R. (2009). Vyroshchuvannia dvolitok koropiv u stavakh za intensyvnoiu tekhnolohiieiu. Kyiv: INKOS.
  3. Haritonova, N. N. (1984). Biologicheskie osnovy intensifikacii prudovogo rybovodstva. Kiev: Naukova dumka.
  4. Hutyi, B. V., et al. (2005). Pokaznyky krovi bychkiv pry khronichnomu nitratno-nitrytnomu toksykozi. Visnyk Dnipropetrovskoho derzhavnoho ahrarnoho universytetu, 246-249.
  5. Krepova, L. V., et al. (2004). Doklinicheskoe izuchenie bezopasnosti fitopreparatov, obladajushhih gepatoprotektornymi svojstvami. Aktual'nye problemy sozdanija novyh lekarstvennyh preparatov prirodnogo proishozhdenija: 8 Mezhdunar. siezd “Fitofarm 2004”, 21-23 ijunja 2004 g.: materialy. Sankt-Peterburg, 111-114.
  6. Nolled, Loud. (2005). Yevropa proty antybiotykiv. Tvarynnytstvo Ukrainy, 2, 19-20.
  7. Karunskyi, O. Y. (2008). Khimichnyi ta aminokyslotnyi sklad amarantu. Visnyk derzhavnoho ahroekolohichnoho universytetu, 2 (23), 190-192.
  8. Kotov, N. T., Miroshnychenko, L. A., & Shatalov, Ye. P. (2012). Kormova kultura – amarant. Suchasni ahrarni tekhnolohii, 10, 14-21.
  9. Haltsev, V. P., Stotskyi, P. I., & Sienik, V. B. (2012). Ohliad zastosuvannia amarantu ta odyn zi sposobiv otrymannia amarantovoi olii, yak dzherela skvalenu. Ahrarnyi visnyk Prychornomoria, 63, 188-191.
  10. Vyshtakalju, A. B. (2000). Fiziologicheskoe sostojanie i produktivnost' kur jaichnogo napravlenija pri skarmlivanii im vitaminno-travjanoj muki iz amaranta. Candidate’s thesis. Kazan'.
  11. Roǔkova, J., Trčkova, M., & Herzig, I. (2004). The use of amaranth grain in diets for broiler chickens and its effect on performance and selected biochemical indicators. Czech Journal Animal Science, 49, 532-541. https://doi.org/10.17221/4341-CJAS 
  12. Pisarikova, B., et. al. (2006). The use of amaranth (genus Amaranthus L.) in the diets for broiler chickens. Veterinarni Medicina, 51, 399-407. https://doi.org/10.17221/5560-VETMED 
  13. Karasova, N. V. (2009). Perspektyvy vykorystannia amarantu. Khranenye y pererabotka zerna, 1, 31-33.
  14. Voronkov, M. F. (2011). Nasinnytstvo novykh sortiv amarantu. Materialy pidsumk. nauk. konf. prof.-vykl. skladu, asp. i zdob. Kharkiv. nats. ahrar. un-tu im. V. V. Dokuchaieva, 11-14 sich. 2011 r. Kharkiv, 60-61.
  15. Alekin, O. A. (1970). Osnovy gidrohimii. Leningrad: Gidrometeoizdat.
  16. Voda rybohospodarskykh pidpryiemstv. Zahalni vymohy ta normy (2006). SOU 05.01–37–385:2006. Standart Minahropolityky Ukrainy. Kyiv: Ministerstvo ahrarnoi polityky Ukrainy.
  17. Pravdin, I. F. (1966). Rukovodstvo po izucheniju ryb. Moskva: Pishhevaja promyshlennost'.
  18. Derviz, G. V., & Vorob'ev, A. I. (1969). Kolichestvennoe opredelenie gemoglobina krovi posredstvom apparata FJeK. Laboratornoe delo, 4, 2-8.
  19. Ivanova, N. T. (1974). Metodika nekotoryh gematologicheskih pokazatelej u ryb. Tipovye metodiki issledovanij produkivnosti vidov ryb v predelah ih arealov: sb. nauchn. tr. Vilnius, 83-90.
  20. Metodiki zootehnicheskih i biohimicheskih analizov kormov, produktov obmena i zhivotnovodcheskoj produkcii (1978).  Dubrovicy: VNIIZh.
  21. Stal'naja, I. D. (1977). Metod opredelenija dienovoj kon'jugacii nenasyshhenyh vysshih zhirnih kislot. Sovremennye metody v biohimii. Moskva, 63-64.
  22. Korobejnikova, E. N. (1989). Modifikacija opredelenija produktov perekisnogo okislenija lipidov v reakcii s tiobarbiturovoj kislotoj. Laboratornoe delo, 7, 8-9.
  23. Dubinina, E. E., Sal'nikova, L. A., & Efimova, L. F. (1983). Aktivnost' i izofermentnyj spektr superoksiddismutazy jeritrocitov i plazmy krovi cheloveka. Laboratornoe delo, 10, 30-33.
  24. Koroljuk, M. A., Ivanova, L. I., & Majorova, I. G. (1988). Metod opredelenija aktivnosti katalazy. Laboratornoe delo, 1, 16-19.
  25. Bradford, M. M. (1976).  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254. https://doi.org/10.1016/0003-2697(76)90527-3 
  26. Plohinskij, H. A. (1969). Rukovodstvo po biometrii dlja zootehnikov. Moskva: Kolos.