Ribogospod. nauka Ukr., 2015; 1(31): 108-119
DOI: https://doi.org/10.15407/fsu2015.01.108
УДК 639.3:597.432

MORPHOPHYSIOLOGICAL PARAMETERS OF SOME STURGEON SPECIES (ACIPENSERIDAE, ACIPENSERIFORMES) OF DIFFERENT AGE GROUPS REARED IN AQUACULTURE

L. Kurovskaya, This email address is being protected from spambots. You need JavaScript enabled to view it. , Schmalhausen Institute of Zoology NAS, Kyiv
V. Lysenko, This email address is being protected from spambots. You need JavaScript enabled to view it. , Schmalhausen Institute of Zoology NAS, Kyiv
S. Neborachek, This email address is being protected from spambots. You need JavaScript enabled to view it. , Schmalhausen Institute of Zoology NAS, Kyiv

Purpose. To study some morphometric parameters, protein and lysozyme content in organs and serum in several sturgeon species reared in aquiculture at the ages of 3, 8 and 18 months, and to investigate the specific and age distribution of lysozyme in fish organs.

Methodology. For the experiments we used sturgeon species of different age groups reared in the fish farm «Fortuna XXI» located in the Galerny Gulf of the Dіnprо river. The study objects were: bester (Huso huso×Acipenser ruthenus), age of 3, 8 and 18 months; Russian sturgeon (Acipenser gueldenstaedtii), age of 3 months, Siberian sturgeon (A. baeri) and sterlet (A. ruthenus), age of 8 month. Following morphometric parameters were determined in the studied fish: weight (g), length (cm), absolute weight of liver, kidneys, spleen (mg), condition factor and relative weight (index, ‰) of organs. The lysozyme content was determined in serum and extracts of the tissues of fish organs by a diffusion method on the agar and protein content was determined by Louri's method.

Findings. We detected significant differences in morphometric parameters, protein and lysozyme content in organs and serum of 3-month bester and Russian sturgeon as well as specific differences of relative organ weight, protein and lysozyme levels in organs and serum of 8-month Siberian sturgeon, bester and sterlet. A comparison of the investigated parameters in 3, 8 and 18-month bester showed an increase in the condition factor with fish growth, significant changes of protein content in fish of different age groups, high level of lysozyme in kidneys and serum of 18-month fish, redistribution of lysozyme contents in fish organs depending on age.

Originality. First comparison of several morphophysiological parameters of 3, 8, 18-month sturgeon species (sterlet, russian sturgeon, siberian sturgeon, bester) reared in aquiculture.

Practical value. Taking into account the high commercial value of sturgeon species, the conducted studies showed that in the conditions of cage culture with the use of live and high quality granulated feeds, juveniles and age 2 commodity sturgeons maintain the high level of general nonspecific immunity inherent to sturgeon species living in natural conditions.

Keywords: sturgeons (Acipenseridae), fish age, morphometric parameters, protein, lysozyme, aquaculture.

REFERENCES

1. Kol'man, G. S., & Kol'man, R. V. (2004). Vliyanie temperatury i pH sredy inkubatsii na bakteriologicheskuyu aktivnost' lizotsima plazmy krovi russkogo osetra (Acipenser gueldenstaedtii Brandt, 1833). Innovatsii v nauke i obrazovanii – 2003: Mezhdunar. nauch. konf., posvyashch. 90-letiyu vysshego rybokhozyaystvennogo obrazovaniya v Rossii. Izvestiya Kaliningradskogo tekhnicheskogo universiteta, 5, 23-27.
2. Kurovskaya, L. Ya. (2013). Lizotsim – biomarker fiziologicheskogo sostoyaniya ryb pri zarazhenii ektoparazitami. Parazitologiya v izmenyayushchemsya mire. Materialy V s’ezda Parazitologicheskogo obshchestva pri RAN: Vseros. konf. s mezhdunar. uchastiem. Novosibirsk, 101.
3. Labinskaya, A. S. (1978). Mikrobiologiya s tekhnikoy mikrobiologicheskikh issledovaniy. Moskva: Meditsina.
4. Lapirova, T. B., & Flerova, E. A. (2013). Sravnitel'nyy analiz nekotorykh immunologicheskikh parametrov krovi shchuki Esox lucius (L.) i sudaka Stizostedion lucioperca (L.). Vestnik Astrakhanskogo gosudarstvennogo universiteta. Seriya: Rybnoe khozyaystvo, 1, 140-145.
5. Lapirova, T. B., Mikryakov, V. R., Mavrin, A. S., & Vinogradov, G. A. (2000). Vliyanie subletal'nykh kontsentratsiy soley rtuti, kadmiya i medi na soderzhanie lizotsima v tkanyakh molodi lenskogo osetra Acipenser baeri. Zhurnal evolyutsionnoy biokhimii i fiziologii, 36(1), 37-39.
6. Stroev, E. A., & Makarova, P. P. (1986). Praktikum po biologicheskoy khimii. Moskva: Vysshaya shkola.
7. Subbotkina, T. A. (1992). Lizotsim syvorotki krovi i leykotsitov russkogo osetra. XІІІ nauch. konf. po ekologicheskoy fiziologii i biokhimii ryb, 2. Petrozavodsk, 121-122.
8. Subbotkina, T. A., & Subbotkin, M. F. (2000). Mezhgodovaya izmenchivost' aktivnosti lizotsima syvorotki krovi sevryugi v razlichnye periody zhiznennogo tsikla. Osetrovye na rubezhe XXІ veka: Mezhdunar. konf. Astrakhan', 197-198.
9. Subbotkina, T. A., & Subbotkin, M. F. (2002). Lizotsim chetyrekh vidov osetrovykh ryb sem. Acipenseridae r. Volgi. Biologiya vnutrennikh vod, 2, 88-93.
10. Subbotkina, T. A., &. Subbotkin, M. F. (2003). Soderzhanie lizotsima v organakh i syvorotke krovi u razlichnykh vidov ryb r. Volgi. Zhurnal evolyutsionnoy biokhimii i fiziologii, 39(5), 430-437.
11. Subbotkina, T. A., & Subbotkin, M. F. (2003). Soderzhanie lizotsima u razlichnykh vidov ryb v zavisimosti ot ikh sistematicheskogo polozheniya. Problemy patologii, immunologii i okhrany zdorov'ya ryb i drugikh gidrobiontov: Vseros. nauch.-prakt. konf. Moskva: Rossel'khozakademiya, 129-130.
12. Subbotkina, T. A., & Subbotkin, M. F. (2007). Uroven' lizotsima u ryb razlichnogo sistematicheskogo polozheniya. Sovremennye problemy fiziologii i biokhimii vodnykh organizmov: ІІ nauch. konf. s uchastiem stran SNG. Petrozavodsk, 149-150.
13. Subbotkina, T. A., & Subbotkin, M. F. (2012). Lizotsim syvorotki krovi ob'ektov akvakul'tury (otryady Cypriniformes i Perciformes) Tsentral'nogo V'etnama. Vestnik Astrakhanskogo gosudarstvennogo universiteta. Seriya: Rybnoe khozyaystvo, 2, 140-147.
14. Hua, Yuping, Liu, Hongbai, & Zhang, Ying. (2005). Levels of a lysozyme in serum and other tissues of yearlings of the Amur sturgeon. Dongbei linye daxue xuebao = Journal Northeast Forestry University, 33(3), 63-66.
15. Lie, O., Evensen, O., Sorensen, A., & Froysadal, E. (1989). Study on lysozyme activity fish species. Diseasеs Aquatic Organisms, 6, 1-5. http://dx.doi.org/10.3354/dao006001
16. Saurabh, Shailesh, & Sahoo, P. R. (2008). Lysozyme: An important defense molecule of fish innate immune system. Aquaculture Research, 39(3), 223-239. http://dx.doi.org/10.1111/j.1365-2109.2007.01883.x
17. Tort, L., Balasch, J. C., & Mackenzie, S. (2003). Fish immune system. A crossroads between innate and adaptive responses. Immunology, 22(3), 277-286.
18. Zheng, Qing-mei, Wu, Rui-quan, & Ye, Xing. (2006). Progress in researches of a lysozyme of aquatic organisms. Shanghai shichan daxue xuebao = Journal Shanghai Fish University, 15(4), 483-487.